福建金线莲与菌根真菌互作过程中的蛋白质组研究(5)
Proteome analysis on interaction between Anoectochilus roxburghii and
Mycorrhizal fungus
GAO Chuan1，2， GUO Shun-xing1*， ZHANG Jing2， CHEN Juan1， ZHANG Li-chun1
(1.Institute of Medicinal Plant Development， Chinese Academy of Medical Sciences and Peking Union
Medical College， Beijing 100193， China；
2.Institute of Pharmaceutical Chemistry of Beijing， Beijing 102205， China)
[Abstract] Objective： To study the mechanism of plant growing promoted by Mycorrhizal fungus through the difference of proteomes. Method： The differential proteomes between uninoculated and inoculated endophytic fungi， Epulorhiza sp. on Anoectochilus roxburghii were analyzed by two-dimensional gel electrophoresis and MALDI-TOF/TOF mass spectrum. Result and Conclusion： Twenty-seven protein spots were analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Twenty-two candidate proteins were identified by database comparisons. The function of these proteins mostly involved in signal transduction， metabolic regulation， as well as photosynthesis and substance metabolism. The results indicate that the regulator control system of plant is influenced by fungi action， and the positive regulation improves substance metabolism and photosynthesis， which results in strong plant and higher resistance. It is also deduced that silent genes may exist in endosymbiosis plants.
[Key words] Anoectochilus； endophytic fungi； proteome； 2D gel electrophoresis
doi：10.4268/cjcmm20122408
[责任编辑 吕冬梅], http://www.100md.com(高川,郭顺星,张靖,陈娟,张丽春)
Mycorrhizal fungus
GAO Chuan1，2， GUO Shun-xing1*， ZHANG Jing2， CHEN Juan1， ZHANG Li-chun1
(1.Institute of Medicinal Plant Development， Chinese Academy of Medical Sciences and Peking Union
Medical College， Beijing 100193， China；
2.Institute of Pharmaceutical Chemistry of Beijing， Beijing 102205， China)
[Abstract] Objective： To study the mechanism of plant growing promoted by Mycorrhizal fungus through the difference of proteomes. Method： The differential proteomes between uninoculated and inoculated endophytic fungi， Epulorhiza sp. on Anoectochilus roxburghii were analyzed by two-dimensional gel electrophoresis and MALDI-TOF/TOF mass spectrum. Result and Conclusion： Twenty-seven protein spots were analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Twenty-two candidate proteins were identified by database comparisons. The function of these proteins mostly involved in signal transduction， metabolic regulation， as well as photosynthesis and substance metabolism. The results indicate that the regulator control system of plant is influenced by fungi action， and the positive regulation improves substance metabolism and photosynthesis， which results in strong plant and higher resistance. It is also deduced that silent genes may exist in endosymbiosis plants.
[Key words] Anoectochilus； endophytic fungi； proteome； 2D gel electrophoresis
doi：10.4268/cjcmm20122408
[责任编辑 吕冬梅], http://www.100md.com(高川,郭顺星,张靖,陈娟,张丽春)